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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
31/01/2017 |
Data da última atualização: |
18/01/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MENEZES, K. A. S.; ESCOBAR, I. E. C.; FRAIZ, A. C. R.; MARTINS, L. M. V.; FERNANDES JUNIOR, P. I. |
Afiliação: |
KELLY ALEXSANDRA SOUZA MENEZES, Universidade do Estado da Bahia, Departamento de Tecnologia e Ciências Sociais; INDRA ELENA COSTA ESCOBAR, UNIVASF; ANA CARLA RESENDE FRAIZ, UNIVASF; LINDETE MÍRIA VIEIRA MARTINS, Universidade do Estado da Bahia, Departamento de Tecnologia e Ciências Sociais, Juazeiro, Bahia; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
Título: |
Genetic variability and symbiotic efficiency of Erythrina velutina Willd root nodule bacteria from the Semi-Arid region in Northeastern Brazil. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Revista Brasileira de Ciência do Solo, v. 41, p. 1-13, 2017. |
Idioma: |
Português |
Conteúdo: |
Legume-rhizobia symbiosis is a cross-kingdom association that results in large mounts of nitrogen incorporated in food webs. For the Brazilian semi-arid region, data on genetic variability and symbiotic efficiency of Papilionoidae rhizobial communities are very scarce. The aim of this study was to evaluate the genetic variability and the symbiotic efficiency of eight rhizobial isolates obtained from ?mulungu? (Erythrina velutina Willd.) nodules. For 16S rRNA gene sequencing, the genomic DNA was extracted using a commercial kit, amplified with universal primers, and subjected to sequencing reactions. For the isolate ESA 71, PCR amplifications for nodC and nodA genes were attempted. Rhizobial efficiency was assessed by two greenhouse experiments. The first assay was carried out under gnotobiotic conditions, with sterile sand as a substrate; the second experiment was conducted in a non-sterile soil. For both experiments, the inoculation treatments consisted of a single inoculation of each isolate, in addition to a treatment with Bradyrhizobium elkanii BR 5609 as a reference strain. Furthermore, two non-inoculated control treatments, supplied and not supplied with mineral N, were also evaluated. Bacterial identification indicated that both and rhizobia could be found in ?mulungu? root nodules. Three isolates where classified within the Rhizobium genus, four bacteria belonged to Bradyrhizobium and one isolate clustered with Burkholderia. Positive amplification of an intragenic fragment of the nodA gene using a primer set to ?-rhizobia could be found for ESA 71 (Burkholderia). All bacterial isolates were effective in colonizing ?mulungu? roots. In the first experiment, all inoculated treatments and N fertilization increased the N concentration in ?mulungu? shoot tissues. For total N in the shoots, the isolates ESA 70, ESA 72, and ESA 75 stood out. In the non-sterile substrate experiment, the isolates ESA 70, ESA 71, ESA 72, and ESA 75, together with the reference strains, induced increases in the shoot N concentration and total accumulation compared to the absolute control. The results indicate that ?mulungu? is able to establish associations with efficient ? and ?-rhizobia in Brazilian semi-arid soils. MenosLegume-rhizobia symbiosis is a cross-kingdom association that results in large mounts of nitrogen incorporated in food webs. For the Brazilian semi-arid region, data on genetic variability and symbiotic efficiency of Papilionoidae rhizobial communities are very scarce. The aim of this study was to evaluate the genetic variability and the symbiotic efficiency of eight rhizobial isolates obtained from ?mulungu? (Erythrina velutina Willd.) nodules. For 16S rRNA gene sequencing, the genomic DNA was extracted using a commercial kit, amplified with universal primers, and subjected to sequencing reactions. For the isolate ESA 71, PCR amplifications for nodC and nodA genes were attempted. Rhizobial efficiency was assessed by two greenhouse experiments. The first assay was carried out under gnotobiotic conditions, with sterile sand as a substrate; the second experiment was conducted in a non-sterile soil. For both experiments, the inoculation treatments consisted of a single inoculation of each isolate, in addition to a treatment with Bradyrhizobium elkanii BR 5609 as a reference strain. Furthermore, two non-inoculated control treatments, supplied and not supplied with mineral N, were also evaluated. Bacterial identification indicated that both and rhizobia could be found in ?mulungu? root nodules. Three isolates where classified within the Rhizobium genus, four bacteria belonged to Bradyrhizobium and one isolate clustered with Burkholderia. Positive amplification of an intragenic fr... Mostrar Tudo |
Palavras-Chave: |
Biological nitrogen fixation; Fixação biológica de notrogenio; Leguminosa arbórea; Planta nativa; Rizóbio; Variabilidade genética. |
Thesagro: |
Caatinga; Mulungu. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154317/1/pAULO-iVAN-2017.pdf
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Marc: |
LEADER 03107naa a2200265 a 4500 001 2062237 005 2018-01-18 008 2017 bl uuuu u00u1 u #d 100 1 $aMENEZES, K. A. S. 245 $aGenetic variability and symbiotic efficiency of Erythrina velutina Willd root nodule bacteria from the Semi-Arid region in Northeastern Brazil.$h[electronic resource] 260 $c2017 520 $aLegume-rhizobia symbiosis is a cross-kingdom association that results in large mounts of nitrogen incorporated in food webs. For the Brazilian semi-arid region, data on genetic variability and symbiotic efficiency of Papilionoidae rhizobial communities are very scarce. The aim of this study was to evaluate the genetic variability and the symbiotic efficiency of eight rhizobial isolates obtained from ?mulungu? (Erythrina velutina Willd.) nodules. For 16S rRNA gene sequencing, the genomic DNA was extracted using a commercial kit, amplified with universal primers, and subjected to sequencing reactions. For the isolate ESA 71, PCR amplifications for nodC and nodA genes were attempted. Rhizobial efficiency was assessed by two greenhouse experiments. The first assay was carried out under gnotobiotic conditions, with sterile sand as a substrate; the second experiment was conducted in a non-sterile soil. For both experiments, the inoculation treatments consisted of a single inoculation of each isolate, in addition to a treatment with Bradyrhizobium elkanii BR 5609 as a reference strain. Furthermore, two non-inoculated control treatments, supplied and not supplied with mineral N, were also evaluated. Bacterial identification indicated that both and rhizobia could be found in ?mulungu? root nodules. Three isolates where classified within the Rhizobium genus, four bacteria belonged to Bradyrhizobium and one isolate clustered with Burkholderia. Positive amplification of an intragenic fragment of the nodA gene using a primer set to ?-rhizobia could be found for ESA 71 (Burkholderia). All bacterial isolates were effective in colonizing ?mulungu? roots. In the first experiment, all inoculated treatments and N fertilization increased the N concentration in ?mulungu? shoot tissues. For total N in the shoots, the isolates ESA 70, ESA 72, and ESA 75 stood out. In the non-sterile substrate experiment, the isolates ESA 70, ESA 71, ESA 72, and ESA 75, together with the reference strains, induced increases in the shoot N concentration and total accumulation compared to the absolute control. The results indicate that ?mulungu? is able to establish associations with efficient ? and ?-rhizobia in Brazilian semi-arid soils. 650 $aCaatinga 650 $aMulungu 653 $aBiological nitrogen fixation 653 $aFixação biológica de notrogenio 653 $aLeguminosa arbórea 653 $aPlanta nativa 653 $aRizóbio 653 $aVariabilidade genética 700 1 $aESCOBAR, I. E. C. 700 1 $aFRAIZ, A. C. R. 700 1 $aMARTINS, L. M. V. 700 1 $aFERNANDES JUNIOR, P. I. 773 $tRevista Brasileira de Ciência do Solo$gv. 41, p. 1-13, 2017.
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Embrapa Semiárido (CPATSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
05/07/1996 |
Data da última atualização: |
21/05/2024 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
MILLAR, A. A.; CORDEIRO, G. G. |
Afiliação: |
AGUSTIN ANTONIO MILLAR, ORGANIZAÇÃO DAS NAÇÕES UNIDAS PARA ALIMENTAÇÃO E AGRICULTURA; GILBERTO GOMES CORDEIRO, CPATSA. |
Título: |
Caracterizacao dos problemas de sais dos setores de irrigacao em operacao do Projeto Sao Goncalo. |
Ano de publicação: |
1978 |
Fonte/Imprenta: |
In: REUNIAO SOBRE SALINIDADE EM AREAS IRRIGADAS, 1978, Fortaleza. Anais... Recife: SUDENE, 1978. |
Páginas: |
p. 25-34. |
Idioma: |
Português |
Conteúdo: |
Trata do Projeto de Irrigacao de Sao Goncalo, Paraiba. Consta de: antecedentes gerais do projeto; metodologia para a definicao dos problemas de sais dos sistemas de irrigacao e problemas de sais nos setores de irrigacao. |
Palavras-Chave: |
Paraiba; Projeto Sao Goncalo. |
Thesagro: |
Drenagem; Irrigação; Salinidade. |
Thesaurus NAL: |
drainage; irrigation; salinity. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/130937/1/ID-7567.pdf
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Marc: |
LEADER 00890nam a2200229 a 4500 001 1132548 005 2024-05-21 008 1978 bl uuuu u00u1 u #d 100 1 $aMILLAR, A. A. 245 $aCaracterizacao dos problemas de sais dos setores de irrigacao em operacao do Projeto Sao Goncalo. 260 $aIn: REUNIAO SOBRE SALINIDADE EM AREAS IRRIGADAS, 1978, Fortaleza. Anais... Recife: SUDENE$c1978 300 $ap. 25-34. 520 $aTrata do Projeto de Irrigacao de Sao Goncalo, Paraiba. Consta de: antecedentes gerais do projeto; metodologia para a definicao dos problemas de sais dos sistemas de irrigacao e problemas de sais nos setores de irrigacao. 650 $adrainage 650 $airrigation 650 $asalinity 650 $aDrenagem 650 $aIrrigação 650 $aSalinidade 653 $aParaiba 653 $aProjeto Sao Goncalo 700 1 $aCORDEIRO, G. G.
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